The Center for Innovations in Medicine (CIM) is offering peptide microarrays and processing for immunosignaturing and other applications. The microarrays consist of peptides spotted or synthesized on standard glass slides or onto slicon dioxide, respectively.
Each peptide is a random sequence. The peptides are exactly 20 amino acids long with a constant GSC linker (for spotted arrays) or ~14 amino acids long with a GSG linker (for in situ synthesized arrays). For spotted arrays, the 17 amino acids are generated by random choice of 19 amino acids (cysteine is excluded) and for in situ synthesized arrays, the 11 amino acids are generated by pseudorandom choice of 18 amino acids (cysteine and methonine are excluded).
In situ synthesized microarrays
The Microarrays are printed on standard 75.4mm x 25mm (3 in x 1 in) glass slides, or synthesized on 8 inch wafers and diced into standard slides. The arrays can be purchased directly for use by the researcher or they can be processed in the Core. Data analysis can be provided. We recommend 10um 2-color scanners for the 10K microarrays and a 0.5um 2-color scanner for the 330K and 350K microarrays. The CIM10K has been used in several different applications including: