Peptide Array Core

The Center for Innovations in Medicine (CIM) is offering peptide microarrays and their processing for several applications. These CIM10K Arrays consist of 10,000 peptides spotted in replicate on standard glass slides.

Each peptide is of a known sequence. The peptides are 20 amino acids long with a constant GSC at the attachment point to the surface. 17 amino acids are generated by random choice of 19 amino acids (cysteine is excluded).

The Microarrays are printed on standard 75.4mm x 25mm (3 in x 1 in) glass slides. Custom arrays can be produced, including subsets of the CIM10K peptides. The Arrays can be purchased directly for use by the researcher or they can be processed in the Core. Data analysis can be provided. The CIM10K has been used in several different applications. These include:

• Immunosignaturing: A small sample of serum, saliva or other fluids can be analyzed for the antibody signature on the array. This procedure is very sample sparing and sensitive to changes in health status. It can be used to characterize vaccines/infections or to develop diagnostics for chronic diseases.

• Ligands: Proteins, viruses, bacteria or cells can be applied to the arrays to find peptide ligands. Conditions for binding can be controlled relative to temperature, solvents or competitors. The Synbody technology provides a method to increase specificity and affinity by linking peptide ligands. Initial leads can be readily improved by a simple maturation procedure.